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Image Search Results
Journal: Immune Network
Article Title: A New Reporter Vector System Based on Flow-Cytometry to Detect Promoter Activity
doi: 10.4110/in.2009.9.6.243
Figure Lengend Snippet: Comparison of the dual reporter system with the conventional CAT reporter system. The same promoter regions used in the dual reporter vector system were re-cloned into the CAT reporter system, pCAT®3-Enhancer vector (A). Vectors harboring different lengths of the promoter region were cotransfected with pSV-β-Galactosidase vector. The transcriptional activities of each region were determined via Chloramphenicol acetyl transferase activity assays (B, C) after compensation with beta-galactosidase. The pCAT3 control vector without the promoter sequence (pCAT®3-Basic) and the one harboring the SV40 promoter (pCAT®3-Control) were used as negative and positive controls, respectively.
Article Snippet: Several promoter regions used in the dual reporter vector were re-cloned into the
Techniques: Plasmid Preparation, Clone Assay, Chloramphenicol Acetyltransferase Assay, Activity Assay, Sequencing